Next Generation Sequencing Interest Group
The Next Generation Sequencing Interest Group Meeting is open to everyone. Investigators that are currently performing or are planning to start studies using the Next Gen Sequencing platforms are encouraged to attend. Please contact Kevin Knudtson, PhD (kevin-knudtson@uiowa.edu) if you have questions about the NGS Interest Group.
Past NGS Interest Group Talks
2021
Tuesday, May 25, 2021
Beyond the DE gene list: understanding your RNA-seq results
Speaker: Michael Chimenti, PhD, Director, IIHG Bioinformatics Division- Tuesday, April 13, 2021
10x Genomics Sample Preparation for Chromium Single Cell Gene Expression, ATAC, and Multiome Solutions
Speakers: Melissa Leone, Senior Sales Executive, 10x Genomics
Mark Gerber, PhD, Senior Field Applications Specialist, 10x Genomics
View this talk on YouTube
- Tuesday, March 16, 2021
Considerations for RNA-Seq and Multi-omics Experiments
Speaker: Claire White, Sr. Sequencing Sales Specialist - Illumina, Inc.
View this talk on YouTube
- Tuesday, February 23, 2021
Genomic Analysis of Chronic Staphylococcus Aureus Infections in Cystic Fibrosis
Speaker: Anthony Fischer, MD, PhD, Assistant Professor of Pediatrics - Pulmonary Medicine, UIHC
View this talk on YouTube
Tuesday, January 19th, 2021: "Interactive Single-Cell RNA-Sequencing Analysis: Insights from the Retina and Beyond" - Andrew Voigt, Graduate sutudent in the Molecular Medicine Graduate Program, Medical Scientist Training Program, Department of Ophthalmology and Visual Sciences
2020
May 12, 2020 - "Exploring the microbial dark matter in the oral cavity using multi-omics" Sukirth M. Ganesan, D.D.S., Ph.D., Assistant Professor, Department of Periodontics, College of Dentistry, University of Iowa
2019
- November 20, 2019 - "Accounting for sources of biological variation in analysis of single cell RNA sequencing experiments" Andrew Thurman, PhD, Assistant Research Scientist, Dept. of Internal Medicine, University of Iowa
- October 16, 2019 - "APP-propriate software for discrete informatic tasks" Rob Piper, PhD, Associate Dean for Research; Director, College of Medicine Core Research Facilities; Professor of Molecular Physiology and Biophysics, Internal Medicine
- September 18, 2019 - "An integrative approach utilizing RNA-Seq, ChIP-Seq, and ATAC-Seq to dissect the oncogenic mechanisms of the TAZ-CAMTA1 and YAP-TFE3 fusion proteins" Munir Tanas, PhD, Assistant Professor, Department of Pathology, University of Iowa
- May 15, 2019 - "Targeted in-situ genome-wide mapping of histone modifications and transcription factors using CUT&RUN analysis." Colin Kenny PhD, Post-Doctoral Researcher, Anatomy and Cell Biology, University of Iowa.
- April 17, 2019 - "Maximize your oncology research and improve low-frequency variant detection using high-quality and flexible NGS strategies" Ashley Dvorak, Director, NGS Field Solutions, Integrated DNA Technologies
"A complete strategy for characterizing on - and off - target CRISPR - Cas9 editing via target enrichment, NGS analysis" Ashley Jacobi, Senior Staff Scientist, Integrated DNA Technologies - March 20, 2019 - "Real time DNA and RNA sequencing using Oxford Nanopore Technologies 'nanopore sensing' platform" Konstantinos (Gus) Potamousis, Oxford Nanopore
- February 20, 2019 - "Biology at True Resolution: Resolving Complex Biology with Single Cell Analysis" Melissa Leone, 10x Genomics
January 2019 - Rescheduled for February
2017
- March 15, 2017 - "The Chromium System: New and Emerging Single Cell Transcriptomic Applications," Chris Black, M.S., Sales Executive – Midwest, 10x Genomics
- February 15, 2017 - "Deciphering role of gut microbiome in the pathogenesis of multiple sclerosis: studies using 16S rRNA gene sequencing," Dr. Ashutosh K. Mangalam, PhD, Assistant Professor of Pathology
January 25, 2017 - "New approaches to Single Cell Analysis using Next Generation Sequencing," Dr. Alasdair I. Reid, PhD, Sr. Sales Specialist - Sequencing & Data Analysis, Ilumina, Inc. - Presentation Slides available.
2016
- November 16, 2016 - "Tcf1 bridges transcriptional and epigenetic regulations of T cell identity", Dr. Hai-Hui (Howard) Xue, MD, PhD, Associate Professor, Department of Microbiology
- October 19, 2016 - "Exploring the interface of microRNA-target interactomes and cardiac disease", Dr. Ryan Boudreau, PhD, Assistant Professor of Internal Medicine, Cardiovascular Division - presentation not available.
- August 17, 2016 - "The Chromium System: Linked Read and Single Cell RNA-Seq Applications Powered by GemCode Technology", Chris Black, MS, 10x Genomics - Presentation Slides or Audio available.
- February 17, 2016 - "NGS of Single Cell RNA-Seq: Cell Type Discovery and Gene Regulatory Network Analysis with PyMINEr", Scott Tyler, PhD Candidate, Molecular and Cellular Biology, Dept of Anatomy and Cell Biology, University of Iowa - available by request only.
January 20, 2016 - "Gaining insights into human disease mechanisms using RNA-seq andother NGS based techniques available at IIHG", Mehdi Keddache, PhD, Sr. Sequencing Specialist, Illumina, Inc.
2015
- December 16, 2015 - "Developing DEEPN as a Powerful new Approach to Study Protein-Protein Interactions Based on Yeast Two-Hybrid Analysis and Next Generation Sequencing", Mark Stamnes, PhD, Associate Professor of Molecular Physiology and Biophysics, University of Iowa - Flyer
- November 11, 2015 - "Transcriptome Analysis Using Hybrid-Seq", Kin Fai Au, PhD, Assistant Professor of Internal Medicine - Pulmonary, University of Iowa
- October 21, 2015 - "Genotype-Phenotype Associations in Immunodeficient Children with 22q11.2 Deletion," Benjamin W. Darbro, MD, PhD, Assistant Professor of Pediatrics, Director, Shivanand R. Patil Cytogenetics and Molecular Laboratory, University of Iowa - Slides only - Flyer
- September 16, 2015 - "Evaluation of Steroid-Induced Interocular Pressure in Cow Eye with RNA-Seq", Terry Braun, PhD, Associate Professor of Biomedical Engineering, Department of Ophthalmology and Visual Sciences Coordinated Laboratory for Computational Genomics Wynn Institute for Vision Research, University of Iowa
- March 18, 2015 - "DNA target enrichment for NGS applications: How To Increase Your Odds for a Successful Outcome", Matt McNeill, PhD, Bioinformatics Scientist, Integrated DNA Technologies
- February 18, 2015 - "What our genes can teach us about preventing suicide", Virginia Willour, PhD, Associate Professor of Psychiatry, University of Iowa
- January 29, 2015, 4-5 PM - "Applications of Single Molecule, Real-Time (SMRT) Sequencing", Jonas Korlach, PhD, Chief Scientific Officer, Pacific Biosciences
January 21, 2015 - "Under-surveyed Variants in Exome Sequencing", Todd Scheetz, MS, PhD, Associate Professor of Ophthalmology and Visual Science, Associate Professor of Biomedical Engineering, University of Iowa
2014
- November 19, 2014 - "Metagenomics Introduction & Method Review", Grant Hill - Sample Prep Specialist, Illumina
- October 15, 2014 - "Large-Scale Exome Sequencing for Inherited Eye Disease", Dr. Adam P. DeLuca, PhD, Postdoctoral Scholar, Steven A. Wynn Institute for Vision Research, University of Iowa
- September 17, 2014 - "Mapping the Transcriptomic Topography of the Human Retina using RNA-Seq", S. Scott Whitmore, MS, PhD Candidate in Genetics, Stephen A. Wynn Institute for Vision Research, University of Iowa
- August 20, 2014 - "Genome Instability in Neuropsychiatric Conditions: Variant Discovery and Interpretation, Dr. Jacob Michaelson, PhD, Assistant Professor of Psychiatry, University of Iowa
- June 18, 2014 - "Gene Targeting Strategies to Solve the Focal Epilepsies", Dr. Michael Hildebrand, Neurogeneticist, University of Melbourne
- May 21, 2014-"Copy Number Variations are Common Cause of Non-Syndromic Hearing Loss", Eliot Shearer, MD, PhD, Postdoctoral Scholar, MORL
- April 10, 2014 - "Clinical Exome Sequencing in Mendelian Disorders: Successes & Pitfalls", Dr. Tony Roscioli, Clinical Geneticist, University of New South Wales, School of Medical Science
- March 19, 2014- "RNA-seq Applications using Illumina sequencing technology," Kim Ferguson - Illumina Sample Prep Specialist
February 12, 2014 - "Exome Sequencing in Pediatrics," Alexander Bassuk, MD, PhD, Pediatrics
2013
- November 20, 2013- "Next Generation Sequencing, the Microbiome and Microbial Communities," John Kirby, PhD, Associate Professor, Microbiology
- September 18, 2013- "Application of NGS and bioinformatics methodologies to aptamer selections," William Thiel, PhD, Postdoctoral Fellow in Giangrande Lab
- August 21, 2013- "Application of RNA-Seq to a mouse model of accelerated heart failure," Kai Tan, PhD, Internal Medicine