10X Chromium Single Cell Features:
- Fast workflow from cell suspension to 3′ or 5’-cDNA libraries, and T or B cell V(D)J analyses.
- Captures 100-80,000+ cells (from up to 8 samples) in < 7 minutes.
- Recovers up to ~65% of cells (typically 50%).
- Low doublet rate (~0.9% per 1,000 cells).
- Compatible with Illumina NovaSeq 6000 and MiSeq sequencers.
- The 10X Single-Cell libraries are most economically sequenced on our Illumina NovaSeq 6000.
- At least 20,000 reads per cell (or nuclei) should be targeted for sequencing.
- It is possible to work with cryo-preserved cells, enabling safe sample shipping and batching.
- The cell size limit is comparatively high. Cells can have a diameter of up to 50 µm.
- In addition to cell suspension samples, nuclei suspensions can also be used. For information regarding sample preparation, please refer to the 10X cell preparation guide.