Library Prep for Illumina Sequencing
NGS Library Prep Fees per Sample
Library | Kit | Campus | Off-campus |
|---|---|---|---|
Illumina mRNA-Seq | Stranded mRNA Prep, Ligation | $137 | $149 |
Illumina mRNA-Seq (to be discontinued) | TruSeq Stranded mRNA | $153 | $170 |
Illumina Total RNA-seq | Stranded Total RNA Prep, Ligation | $182 | $194 |
Illumina Total RNA-seq (to be discontinued) | TruSeq Stranded Total RNA Gold | $214 | $230 |
KAPA DNA Seq | KAPA HyperPrep (WGS, Other) | $129 | $141 |
Agilent Exome Capture, 1-16 samples | Sure Select, v5 or v6+UTR baits | $355 | $408 |
Agilent Exome Capture, 17+ samples | Sure Select, v5 or v6+UTR baits | $300 | $345 |
Illumina smRNA-seq | TruSeq smRNA | $282 | $330 |
ChIP-Seq | TBD | inquire | inquire |
Low coverage WGS (genotyping by sequencing) | TBD | inquire | inquire |
Genome Sequencer Fees
Illumina NovaSeq 6000
Illumina MiSeq
flow cell/run type | Reads (Clusters) | Yield | Campus Rate | Off-Campus Rate |
|---|---|---|---|---|
| MiSeq Standard Flow Cell | ||||
50 Cycle (50 bp SR / 25 bp PE) | 12-15 M | 750-850 Mb | $1,209 | $1,259 |
150 Cycle (150 bp SR / 75 bp PE) | 22-25M | 3.3-3.8 Gb | $1,417 | $1,517 |
300 Cycle (300 bp SR / 150 bp PE) | 12-15M | 4.5-5.1 Gb | $1,733 | $1,833 |
500 Cycle (250 bp PE) | 12-15M | 7.5-8.5 Gb | $1,989 | $2,189 |
600 Cycle (300 bp PE) | 22-25 M | 13.2-15 Gb | $2,463 | $2,663 |
| MiSeq Micro Flow Cell | ||||
300 Cycle (300 bp SR / 150 bp PE) | 4M | 1.2 Gb | $665 | $715 |
| MiSeq Nano Flow Cell | ||||
300 Cycle (300 bp SR / 150 bp PE) | 1M | 300 Mb | $487 | $537 |
500 Cycle (250 bp PE) | 1M | 500 Mb | $557 | $607 |
Contact Information:
116 EMRB
Phone: (319) 335-6736
Fax: (319) 335-6737
dna-genomeseq@uiowa.edu
For information about downstream bioinformatics support, contact the IIHG Bioinformatics Division at (319) 335-6717.