The University of Iowa

Carver College of Medicine

Iowa Institute of Human Genetics

NGS Library Prep Fees (per sample)

effective March 1, 2025

Library	Kit	Campus	Off-campus
Illumina mRNA-Seq	Stranded mRNA Prep, Ligation	$137	$149
Illumina Total RNA-seq	Stranded Total RNA Prep, Ligation	$182	$194
KAPA DNA Seq	KAPA HyperPrep (WGS, Other)	$129	$141
Agilent Exome Capture, 1-16 samples	Sure Select, v5 or v6+UTR baits	$355	$408
Agilent Exome Capture, 17+ samples	Sure Select, v5 or v6+UTR baits	$300	$345
Illumina smRNA-seq	TruSeq smRNA	$287	$335
ChIP-Seq	TBD	inquire	inquire
Low coverage WGS (genotyping by sequencing)	TBD	inquire	inquire

Genome Sequencer Fees

effective March 1, 2025

Illumina NovaSeq 6000

flow cell/ run CONFIGURATION	~READS/LANE	~YIELD/LANE	UI Cost per Lane	External Cost per Lane	UI Cost per Flow Cell	External Cost per flow cell
SP (2 lane flow cell)
1x100 bp SR	400M	40 GB	$1,979	$2,148	$3,325	$3,663
2x50 bp PE	400M	40 GB	$1,979	$2,148	$3,325	$3,663
2x100 bp PE	400M	80 GB	$2,515	$2,762	$4,396	$4,890
2x150 bp PE	400M	120 GB	$2,813	$3,138	$4,993	$5,643
2x250 bp PE	400M	200 GB	$3,849	$4,343	$7,065	$8,053
S1 (2 lane flow cell)
1x100 bp SR	800M	80 GB	$3,016	$3,185	$5,399	$5,737
2x50 bp PE	800M	80 GB	$3,016	$3,185	$5,399	$5,737
2x100 bp PE	800M	160 GB	$3,759	$4,006	$6,885	$7,379
2x150 bp PE	800M	240 GB	$4,146	$4,471	$7,659	$8,309

flow cell/ run CONFIGURATION	~READS/LANE	~YIELD/LANE	UI Cost per Lane	External Cost per Lane	UI Cost per Flow Cell	External Cost per flow cell
S2 (2 lane flow cell)
1x100 bp SR	2,000M	200 GB	$5,473	$5,681	$9,946	$10,362
2x50 bp PE	2,000M	200 GB	$5,473	$5,681	$9,946	$10,362
2x100 bp PE	2,000M	400 GB	$6,736	$7,061	$12,473	$13,123
2x150 bp PE	2,000M	600 GB	$7,367	$7,835	$13,734	$14,670
S4 (4 lane flow cell)
2x100 bp PE	2,500M	500 GB	$5,571	$6,039	$19,699	$21,571
2x150 bp PE	2,500M	750 GB	$6,214	$6,786	$22,274	$24,562
1x35 bp SR	2,500M	85 GB	$4,532	$4,961	$15,546	$17,262

Illumina MiSeq

flow cell/run CONFIGURATION	Reads (Clusters)	Yield	Campus Rate	Off-Campus Rate
MiSeq Standard Flow Cell
50 Cycle (50 bp SR / 25 bp PE)	12-15 M	750-850 Mb	$1,254	$1,304
150 Cycle (150 bp SR / 75 bp PE)	22-25M	3.3-3.8 Gb	$1,467	$1,567
300 Cycle (300 bp SR / 150 bp PE)	12-15M	4.5-5.1 Gb	$1,791	$1,891
500 Cycle (250 bp PE)	12-15M	7.5-8.5 Gb	$2,053	$2,253
600 Cycle (300 bp PE)	22-25 M	13.2-15 Gb	$2,547	$2,747
MiSeq Micro Flow Cell
300 Cycle (300 bp SR / 150 bp PE)	4M	1.2 Gb	$689	$739
MiSeq Nano Flow Cell
300 Cycle (300 bp SR / 150 bp PE)	1M	300 Mb	$503	$553
500 Cycle (250 bp PE)	1M	500 Mb	$576	$626

Contact Information:

116 EMRB
Phone: (319) 335-6736
Fax: (319) 335-6737
dna-genomeseq@uiowa.edu

For information about downstream bioinformatics support, contact the IIHG Bioinformatics Division at (319) 335-6717.