Sanger Sequencing
The IIHG Genomics Division houses 2 Applied Biosystems Model 3730xlu (96-capillary) DNA Sequencers. Sequencing can be performed on single-stranded, double-stranded or PCR-generated templates.
Users must first set up an on-line profile and account with the IIHG Genomics Division. Once an account has been established, Sanger sequencing requests should be made through the on-line ordering system.
Please review the sample submission guidelines prior to submitting a request. The user should print their requests and bring the completed request form along with the samples to the division. Tubes must be labeled with 5-digit order number and sample number.
Users will receive email notification when their DNA sequencing results are ready (usually 24 to 48 hours). The email will contain a reminder to login to your account to access the sequencing results that can be downloaded via a web browser through our website. Access to the data files is password protected. This system is NOT HawkID based. You may create or look at your account using the on-line ordering system.
Links to publicly available DNA Sequencing chromatogram trace viewers can be found here.
Contact Information
116 EMRB
Phone: (319) 335-6736
Fax: (319) 335-6737
dna-sequencing@uiowa.edu
Samples for sequencing may be dropped off at 116 EMRB anytime from 8:00 AM to 5:00 PM Monday-Friday.