Sequencers
Illumina NovaSeq6000 Sequencing System
The IIHG Genomics Division currently operates the Illumina NovaSeq 6000 sequencing platform. The NovaSeq 6000 is a two flow cell instrument that is capable of accommodating a variety of run formats which deliver varying sequencing yields, depending on the need of the project (see table below). The ability to run two flow cells concurrently also ensures that priorities for both clinical and research samples are maintained.
As noted in the table below, there are 4 different flow cell options: SP, S1, S2, and S4. Each flow cell has two lanes, except for the S4 which has four lanes. Each flow cell type gives a different number of reads per lane and the resulting yield depends on the run format (read length). Samples are barcoded (indexed) during library preparation to permit the ability to load multiple samples per lane. Please contact the Genomics Division if you have questions about the depth of coverage needed for your project, the most suitable flow cell and run format for your project and how many samples you could combine per lane.
NovaSeq 6000: Approximate Yield/Flow Cell Lane | ||||
Flow Cell Type (Approximate Reads/Lane) | ||||
Run Format | SP(325-400 M) | S1(650-800 M) | S2(1,650-2,000 M) | S4(2,000-2,500 M) |
1 x 100 bp | 40 Gb | 80 Gb | 200 Gb | NA |
2 x 50 bp | 40 Gb | 80 Gb | 200 Gb | NA |
2 x 100 bp | 80 Gb | 160 Gb | 400 Gb | 500 Gb |
2 x 150 bp | 120 Gb | 240 Gb | 600 Gb | 750 Gb |
2 x 250 bp | 200 Gb | NA | NA | NA |
| NA = Not Available | ||||
Illumina MiSeq Sequencing System
The Illumina MiSeq is a “benchtop” sequencer that uses a single lane flow cell capable of yielding one million to 25 million reads per run depending on flow cell type and run format. The run time ranges from a few hours to three days. This instrument is recommended for projects that require fast turn-around time but do not require a great number of reads.
MiSeq Reagent Kit v2 | MiSeq Reagent Kit v3 | ||||||
| Read Length | 1 x 36 bp | 2 x 25 bp | 2x150 bp | 2 x 250 bp | 2 x 75 bp | 2 x 300 bp | |
| Total Time | ~4 hrs | ~5.5 hrs | ~24 hrs | ~39 hrs | ~21 hrs | ~56 hrs | |
| Output | 540-610 Mb | 750-850 Mb | 4.5-5.1Gb | 7.5-8.5 Gb | 3.3-3.8 Gb | 13.2-15 Gb | |
Contact Information
116 EMRB
Phone: (319) 335-6736
dna-genomeseq@uiowa.edu
For information about downstream bioinformatics support, contact the IIHG Bioinformatics Division at (319) 335-6717.